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Agarose Gel Electrophoresis Competition

Event Date and Time: 
Tue, 08/17/2010 - 10:00am - 4:00pm
Location: 
Royal Roads University

Agarose gel electrophoresis is a method used in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, or to separate proteins by charge.[1] Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through an agarose matrix. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.[2] Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins.